The EPR measurements showed that the superhyperfine splitting is anisotropic and that the number of observable lines (17 at 9 Gc/sec along ) and the splitting of the lines is field-dependent. Superhyperfine splitting 9 1.2.6. The greater the covalency, the greater is the hyperfine splitting. 1.2. IH= 1/2, so 2(4)(1/2) + 1 = 5 So, spending most time on N’s, less on H. Superhyperfine coupling overlapping pentet of pentets. The structure observed in the perpendicular region of the spectra is due to nitrogen superhyperfine splitting of the ligands. Triplet: hyperfine splitting. 19. The phenomena of saturation and relaxation 10 1.3.1. It is possible for the unpaired electron to spend differing amounts of time on different nuclei. If we are dealing with a single crystal, then there is only one EPR line (all molecules have the same orientation), which might be split up by (super)hyperfine … ESR METHODS OF STUDY FREE RADICALS AND ANTIOXIDANTS 15 2.1. The resolved super-hyperfine splitting of anisotropic ESR spectra of both studied compounds was firstly observed. Doublet: superhyperfine splitting. vealed that the superhyperfine splitting of the g, ESR signal of ferrousNO complexes is dependent on the conformation of the protein and thus the interaction of the axial ligands with the heme iron. Ligand superhyperfine splitting in the low pH form of the esr signal of Cu(II)-dog albumin were not resolved. Spin-spin interaction of electrons 10 1.3. The value of 14 × 10 −4 cm −1 and the presence of three peaks for the nitrogen superhyperfine structure of the complex are in accordance with expectations for two N donors per copper(II) ions [52, 53]. The electron spin resonance (ESR) spectra of niobocene and vanadocene dichlorides were studied on the title compounds prepared as magnetically diluted species in polycrystalline form. Triplet: hyperfine splitting. Spin - lattice Relaxation 12 1.3.3. In contrast, the esr spectra of Vu(II)-dog serum albumin complex showed a transition from a low pH form to a high pH form as the pH was increased to 9.5. Superhyperfine splitting of the EPR spectra in this system was previously observed only at the orientation of the magnetic field B along the symmetry axis of the crystal [3]. Octet It is possible for the unpaired electron to spend differing amounts of time on different nuclei. Superhyperfine Splitting Septet. Superhyperfine Splitting Examples: Sextet Septet Octet Superhyperfine splitting is direct evidence for COVALENCY! interaction) or with the nucleus of a ligand (superhyperfine interaction), is of no concern for the simulation, since they are treated in the same way. IF= ½, so 2(6)(1/2) + 1 =7 Triplet: superhyperfine splitting.IN= 1, so 2(1)(1) + 1 = 3 So, spending most time on F’s, less on N. Nonet: hyperfine splitting. Examples: Sextet Superhyperfine splitting is direct evidence for COVALENCY! 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